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Fig. 2 | Genome Biology

Fig. 2

From: Transgenerational effect of mutants in the RNA-directed DNA methylation pathway on the triploid block in Arabidopsis

Fig. 2

Transgenerational change of non-CG DNA methylation in inbred generations of nrpd1 mutants. a Scheme of defining three groups of differentially methylated regions (DMRs): wild type (wt), first-generation homozygous nrpd1 (F2), and inbred nrpd1 (Fi). b, c Venn diagrams showing the overlap of CHG DMRx and DMR1 (b) and CHH DMRx and DMR1 (c). d, e Venn diagrams showing the overlap of CHG DMR1 and DMRi (d) and CHH DMR1 and DMRi (e). DMR1spec refers to DMR1 not overlapping with DMRi. f Heatmaps of fractional CHG and CHH DNA methylation levels at DMR1spec and DMRi loci in wt, F2, and Fi nrpd1 mutants. g Boxplots of fractional CHG and CHH DNA methylation levels at DMR1spec and DMRi loci in wt, F2, and Fi nrpd1 mutants. Boxes show medians and the interquartile range, and error bars show the full range excluding outliers. *p < 0.05, ***p < 0.001; n.s, not significant (Wilcoxon test)

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