Fig. 4

Elevated Tet1 is essential for MB progression. a, b Kaplan–Meier curves show the significant increase in survival from SmoA1^+/+ mice crossed with hemizygous deletion of Tet1 (a p < 0.0001; log-rank test), but not crossed with hemizygous deletion of Tet2 (b p = 0.5830; log-rank test). c, d 5hmC dot blot analysis shows significant increase in 5hmC levels in tumors from SmoA1^+/+;Tet1^+/− mice (n = 5; 3 representative samples shown) compared to tumors from SmoA1^+/+ (n = 5; 3 representative samples shown) despite similar ages of tumor-associated symptoms shown in box plot below dot blot (n = 5 per group). e Tet1 protein expression is significantly higher in SmoA1-MBs (n = 7) compared to corresponding NCs (n = 4) (p < 0.05). f Pearson correlation between Tet1 expression and age-of-onset (Pearson R² = 0.5059, *p = 0.0366). g H&E staining (left) and ratio per phenotype (right) of 12-week-old SmoA1^+/+ mice in the presence of either wild-type or hemizygous deletion of Tet1 (p < 0.0001; Welch’s t-test). h Fluorescence microscopy of normal cerebellum and MB with Ki67 (red) and Tet1 (green) in 12-week-old SmoA1^+/+ mice showing Tet1 expression is significantly higher in Ki67-positive cells (blue: DAPI, **** p < 0.001)