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Fig. 6 | Genome Biology

Fig. 6

From: PEPPRO: quality control and processing of nascent RNA profiling data

Fig. 6

Run-on efficiency is measured with pause indices. a Schematic demonstrating pause index calculation. b Pause index values for Drosophila melanogaster GRO-seq libraries with (GSM577247) or without sarkosyl (GSM577248). c The histogram of pause index values is shifted to the right upon addition of sarkosyl in GRO-seq libraries. d Pause index values for test set samples (Values above the dashed line are recommended). e High pause index identified in H9 treated PRO-seq. f Low pause index from HelaS3 GRO-seq

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