Fig. 5
From: PEPPRO: quality control and processing of nascent RNA profiling data
Nascent RNA purity is assessed with the exon-intron ratio. a Schematic demonstrating mRNA contamination calculation. redX represents the exclusion of the first exon in the calculation. b Median mRNA contamination metric for test set samples (shaded region represents recommended range (1-1.8)). c Histogram showing the distribution of mRNA contamination score across genes in the K562 PRO-seq sample. d As in panel c for a GRO-seq library. e mRNA contamination distribution for K562 PRO-seq spiked with 30% K562 RNA-seq. f mRNA contamination distribution for HelaS3 GRO-seq is comparable to the 30% RNA-seq spike-in sample