Fig. 5
From: Drosophila primary microRNA-8 encodes a microRNA-encoded peptide acting in parallel of miR-8
Uncoupled activity of miR-8 and miPEP-8. a Rescue assay of miR-8- or miPEP-8-induced wing phenotype in flies co-over-expressing miR-8 or miPEP-8 along with a miR-8 sponge (miR-8sp) or a miR-8 scramble (miR-8scr). Only miR-8-sp (and not miR-8scr) compensates for miR-8-induced wing size reduction, hence efficiently titrating miR-8, while it has no effect on miPEP-8-induced wing phenotype. b Quantification of a. “ctrl” (MS1096/+) n = 19; “mir-8; mir-8scr” n = 20; “mir-8; mir-8sp” n = 21; “miPEP-8; mir-8scr” n = 23; “miPEP-8; mir-8sp” n = 19. * p < 0.05