Fig. 4

targeting miPEP-8 in vivo in Drosophila induces a wing phenotype. a Strategy for endogenous miPEP-8 edition. The pri-miR-8 gene region was deleted by CRISPR and a P landing site was created. Wild type and miPEP-8 ATG mutated pri-miR-8 in pattB were inserted at the P landing site. b Similar rescue efficiency was observed in at least three independent transgenic lines (left panel). qPCR on mature miR-8 in wild type and mutant (mt) pri-miR-8 Knock In (KI) lines showed similar miR-8 levels (n = 4), (right panel). c Wing phenotype in miR-8 deletion edited line. The pri-miR-8 miPEP-8 mutated (mt) shows a reduced wing size compared to the wild type pri-miR-8. (n = 15 and 28 respectively). d–f Analyses in miPEP-8 mutant identified in DGRP polymorphism. d miR-8 level determined by qPCR in white recipient flies (w) and in white flies carrying the miPEP-8 truncated form (miPEP-8alt), (n = 6 and 8, respectively). e, f Wing size determination in different genetic contexts. miPEP-8alt homozygotes or over miR-8 deficiencies revealed significant reduced wing size relative to the white recipient flies (w, n = 19; miPEP-8alt, n = 21; miPEP-8alt/miR-8 deletions, n = 40). Expressing miPEP-8 rescued the wing phenotype of miPEP-8alt flies relative to sibling flies not expressing miPEP-8 (n = 18 and 28, respectively). Significant (*) or nonsignificant (ns) differences are indicated either relative to white recipient flies or between the two groups