Fig. 2

Drosophila miPEP-8 is biologically active during development. a Lethality assay on flies over-expressing miR-8, miPEP-8 or miPEP-8mt (ATG mutated) using the miR-8 GAL4 driver. Left: details of the genetic cross and expected percentage depending on the effect (neutral, deleterious or advantageous) on Drosophila development. Right: graph indicating the percentage of hatched flies over-expressing the different constructs. White flies (w) crossed with the driver line were used as a control. Expressing miR-8 resulted in developmental lethality since less than 20% of flies hatched (expected value 50%). A significant decrease occurred following miPEP-8 over-expression but not with the untranslatable miPEP-8mt construct. Number of independent crosses: for w and miR-8 n = 23; for miPEP-8 wt and mt n = 24. b Same as in a except the constructs were expressed in wings using the MS1096 driver and the phenotypes scored on wing size. Number of wings analyzed: for w n = 20; for miR-8 n = 27; for miPEP-8 wt and mt n = 27. * or ns: Significant differences are indicated relative to white recipient flies. AU arbitrary units