Fig. 2
HDAC4 is required for senescence escape. a Immunoblot analysis in BJ/hTERT/E1A/RAS/HDAC4+/+ or −/− cells, as indicated. Actin was used as loading control. b Analysis of the senescent cells as scored after SA-β-gal staining. Mean ± SD; n = 3. c RNA-expression levels of the indicated genes in BJ/hTERT/E1A/RAS/HDAC4−/− with respect to WT cells. Mean ± SD; n = 3. d Immunoblot analysis in BJ/hTERT cells expressing the indicated transgenes. Where indicated, HRASG12V was induced for 9 days. Anti-GFP was used to visualize GFP-NFKBIA. Actin was used as loading control. e Analysis of the senescence as scored after SA-β-gal staining in BJ/hTERT cells expressing the indicated transgenes. f Analysis of the population doublings in BJ/hTERT cells expressing the indicated transgenes, scored during the 9 days of observation. g RNA-expression levels of the indicated genes in the cells co-expressing RAS and the indicated transgenes, with respect to HYGRO expressing cells not expressing RAS. Mean ± SD; n = 3. The significance is relative to RAS+HygroR co-expressing cells