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Fig. 6 | Genome Biology

Fig. 6

From: Functional mapping of androgen receptor enhancer activity

Fig. 6

SNVs impact AR enhancer activity. a An increase in SNVs at ARBS is observed in both primary (left) and metastatic (right) PCa. b The impact of clinical SNV on androgen-dependent enhancer activity was quantified with a luciferase assay at inducible ARBS. Those SNVs that significantly altered AR enhancer activity (3/16) are shown (4 biological replicates ± SEM; *p < 0.05, ***p < 0.001). c Genome browser snapshot of ZBTB16 gene locus. Gene looping is observed between enhancer ARBS_490 and ZBTB16 promoter. d Expression of ZBTB16 was quantified by qPCR after CRISPRi inhibition of ARBS_490. Androgen-induced expression of ZBTB16 is suppressed compared to non-target (NT) gRNA control (3 biological replicates ± SEM; **p < 0.01)

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