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Fig. 7 | Genome Biology

Fig. 7

From: PD-L1 lncRNA splice isoform promotes lung adenocarcinoma progression via enhancing c-Myc activity

Fig. 7

Binding of PD-L1-lnc with c-Myc enhances the association of c-Myc with Max and promotes c-Myc nuclear translocation. a Graphical representation of three-dimensional structures of PD-L1-lnc and c-Myc docking models with a zoom-in image of the binding interface. The binding region was shown in three different visualizations (cartoon and sphere). b Schematic representation of the PD-L1-lnc del vector with the predicted binding sites removed and the PD-L1-lnc mut vector which was mutated at the predicted binding sites (marked red). c Pulldown of PD-L1-lnc in A549 cells transfected with WT or mutant PD-L1-lnc/GFP vector using biotinylated antisense probes against GFP mRNA to detect the binding of PD-L1-lnc with c-Myc. d Immunoprecipitation of c-Myc in A549 cells transfected with WT or mutant PD-L1-lnc/GFP vector using anti-c-Myc antibody to assess the binding of PD-L1-lnc with c-Myc. e Binding of PD-L1-lnc with c-Myc resulted in recruitment of Max by c-Myc in A549 cells. f, g Immunofluorescence (f) and Western blot analysis (g) of c-Myc distribution in A549 cells with or without PD-L1-lnc overexpression or knockdown. Note that PD-L1-lnc overexpression and PD-L1-lnc knockdown markedly increases and decreases the nuclear distribution of c-Myc in A549 cells, respectively. *P < 0.05;**P < 0.01. Scale bar, 10 μm

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