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Fig. 2 | Genome Biology

Fig. 2

From: G-quadruplexes are transcription factor binding hubs in human chromatin

Fig. 2

TFs selectively bind to G4 structures. a Affinity pull-down western blot analysis of different G4 oligonucleotides and control sequences. Genomic enrichment at endogenous G4s in K562 for randomization in open chromatin is shown in brackets. b Affinity pull-down of SP2, FUS, and NRF1 using a G4 oligomer, single-stranded oligomers unable to form a G4 structure (ss mutMyc and ss Myc*) and respective consensus sequences. c Binding curves as determined by ELISA show high-affinity binding of recombinant FLAG-NRF1 to a NRF1 double-stranded DNA consensus sequence and G4 structures, but significantly weaker binding to a single-stranded 7-deaza control (error bars display standard deviation, N = 3)

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