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Fig. 5 | Genome Biology

Fig. 5

From: Identification of X-chromosomal genes that drive sex differences in embryonic stem cells through a hierarchical CRISPR screening approach

Fig. 5

Global transcriptome profiling of Klhl13 and Dusp9 heterozygous mutant lines. a Differentially expressed autosomal genes (DEGs) in XO (gray), D9-HET (yellow), K13-HET (blue), and D9K13-HET cells (red) compared to the parental XX line were identified by RNA-seq (log2(fold-change) > 0.5 or log2(fold-change) < − 0.5, p value < 0.05). The overlap between these gene sets is shown in Venn diagrams. b Principal component analysis (PCA) of the 100 most variable autosomal genes across XX (black), XO (gray), and heterozygous mutant cell lines (D9 yellow, K13 blue, and D9K13 red), averaged across three replicates

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