Fig. 3
ddPCR and WES concordance: a VAF concordance of individual cell line WES consensus results with ddPCR assays of that cell line. b Concordance (log10 scale) of Sample A VAF between ddPCR and WES for positives only. c Various dilutions (C, D, E) of Sample A into B achieve the expected reduction in VAF as seen in the ddPCR results. It also shows the potential noise for measuring ddPCR variants below 0.1% (10− 3) in the distribution of Sample B variants. d Concordance of replicate ddPCR assays (on log10 scale) is very high (r2 = .95) in diluted target Samples D and E. Putative VAF values from Sample B are also shown for comparison