Fig. 5

Coefficient of variation (CV) of TMB. a Technical run-to-run variance of TMB with VAF cutoff above 2.5% was estimated for six panels at different TMB levels. A power-law curve (dashed line) is fitted for each panel. b Technical run-to-run variance of TMB with VAF cutoff above 5%. c The intrinsic CV is plotted with the equation (embedded, see “Methods” for detail) for each panel based on their panel size. The curve (dashed) for size of 1 Mb is also plotted as a reference. d The overall CV is plotted combining technical and intrinsic CV. The solid fitting power-law curve is for TMB (VAF > 2.5%), and the dashed curve is for TMB (VAF > 5%)