Fig. 1
From: Prevention of acquired sensorineural hearing loss in mice by in vivo Htra2 gene editing
In vitro genome editing with the SpCas9 therapeutic system. a Design of the gRNAs. The three gRNA targeting sequences in the Htra2 gene are listed. PAM sites are marked by red nucleotides. The red arrows indicate the exons targeted by the gRNAs, and the gray arrows indicate the primers. b The indel frequency of the gRNAs was tested in HEI-OC1 cells using high-throughput sequencing. Dots represent individual values and bars represent the mean ± SD, n = 2. c Indels causing in-frame versus frameshift mutations (percentages are shown) in the coding sequence after transfection with SpCas9–gRNA. d Indel profiles from HEI-OC1 cells transfected with Sp-g1, Sp-g2, and Sp-g3, respectively. Negative numbers represent deletions, and positive numbers represent insertions. nt nucleotides