Fig. 7
PER1 and TP53 may serve as the key candidate gene of YTHDF2. a Bioinformatics analysis filtered PER1 and TP53 may serve as potential downstream target of YTHDF2. b, c Gene expression correlation analysis from the TCGA database indicated that YTHDF2 expression negatively correlated with PER1 (R = -0.417, P = 1.19e−04) (b) and TP53 (R = − 0.609, P = 1.98e−09) (c). d, e IGV tracks for PER1 (d) and TP53 (e) from MeRIP-seq analysis in ocular melanoma cells. f, g MeRIP-qPCR revealed m6A modification of PER1 (f) and TP53 (g) mRNAs. h, i RIP-qPCR analysis revealed that YTHDF2 enriched in PER1 and TP53 transcripts in OCM1 (h) and CRMM1 (i) cells. j Expression of PER1 and TP53 was detected in OCM1 and CRMM1 cells with YTHDF2 knockdown by Western blot. k Expression of PER1 and TP53 was detected in OCM1 and CRMM1 cells treated with different concentrations of Nala for 24 h by Western blot