Fig. 5

YTHDF2 acted as an oncogene in ocular melanoma cells. a, b RT-PCR (a) and Western blot (b) showed that YTHDF2 was silenced after shRNAs transfection in ocular melanoma cells. **p < 0.01. c, d Proliferation of OCM1 (c) and CRMM1 (d) cells with or without YTHDF2 knockdown was analyzed by CCK8 assay. ***p < 0.001, ****p < 0.0001. e Tumor growth of OCM1 and CRMM1 cells with or without YTHDF2 knockdown was evaluated by colony formation assay. f Statistical analysis of the colony formation assay performed using OCM1 and CRMM1 cells with or without YTHDF2 knockdown. All of the experiments were performed in triplicate, and relative colony numbers are shown as means ± SD. **p < 0.01. g The migratory ability of OCM1 and CRMM1 cells with or without YTHDF2 knockdown was evaluated by transwell assay. h Statistical analysis of cells in the transwell assay performed using OCM1 and CRMM1 cells with or without YTHDF2 knockdown. All of the experiments were performed in triplicate, and relative cell numbers are shown as means ± SD. **p < 0.01