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Fig. 4 | Genome Biology

Fig. 4

From: Histone lactylation drives oncogenesis by facilitating m6A reader protein YTHDF2 expression in ocular melanoma

Fig. 4

Aberrant YTHDF2 expression in ocular melanoma correlated with prognosis. a IGV tracks for YTHDF2 expression from RNA-sequence analysis normal cell lines (PIG1 and RPE) and ocular melanoma cell lines (OCM1 and OM431). b Expression of YTHDF2 was detected in normal melanocytes and ocular melanoma cell lines by RT-PCR. *p < 0.05, **p < 0.01. c Expression of YTHDF2 was detected in normal melanocytes and ocular melanoma cell lines by Western blot. d Densitometric analysis was performed to quantify and statistically compare YTHDF2 levels in normal melanocytes and ocular melanoma cell lines that normalized to β-Actin. *p < 0.05, **p < 0.01. e, f YTHDF2 expression in normal and tumor tissues. e YTHDF2 expression was visualized by immunofluorescence staining in normal and tumor tissues. Scale bar: left panel, 100 μm; right panel, 20 μm. b Statistical results of YTHDF2 expression in normal and tumor tissues. Unpaired t test, **p < 0.01. g Kaplan-Meier curves of time for recurrence showing the difference between ocular melanoma patients with low and high YTHDF2 expression. “High YTHDF2” was defined as relative YTHDF2 expression level of the samples (determined by luminance intensity) higher than 0.72, while those lower than 0.72 as “low YTHDF2.” n = 55, log-rank test, p < 0.05. h YTHDF2 expression in patients at AJCC stages T1 to T4. There were 2 ocular melanoma samples in T1 stage, 36 in T2 stage, 42 in T3 stage, and 2 in T4 stage. i The TCGA database of uveal melanoma demonstrated prolonged survival time in patients with low YTHDF2 expression

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