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Fig. 1 | Genome Biology

Fig. 1

From: Histone lactylation drives oncogenesis by facilitating m6A reader protein YTHDF2 expression in ocular melanoma

Fig. 1

Ocular melanoma exhibited increased lactyation levels and associated with poor survival in UVM patients. a, b Lactyation levels in normal and tumor tissues. a Lactyation levels were visualized by immunofluorescence staining in normal and tumor tissues. Scale bar: left panel, 100 μm; right panel, 20 μm. b Statistical results of lactyation levels in normal and tumor tissues. Unpaired t test, ** p < 0.01. c Kaplan-Meier curves of time for recurrence showing the difference between ocular melanoma patients with low and high lactyation levels. “High lactylation” was defined as relative lactylation level of the samples (determined by luminance intensity) higher than 0.87, while those lower than 0.87 as “low lactylation”. n = 56, log-rank test, p < 0.05. d Lactyation levels in patients at AJCC stages T1 to T4. There were 3 ocular melanoma samples in T1 stage, 36 in T2 stage, 40 in T3 stage, and 3 in T4 stage. e Lactyation and H3K18la levels were detected in normal melanocytes and ocular melanoma cell lines by Western blot. f Densitometric analysis was performed to quantify and statistically compare lactyation levels in normal melanocytes and ocular melanoma cell lines that normalized to histone H3. *p < 0.05, **p < 0.01. g Silver staining-mass spectrometry (MS) of lactylated proteins. h Densitometric analysis was performed to quantify and statistically compare H3K18la levels in normal melanocytes and ocular melanoma cell lines that normalized to histone H3. *p < 0.05, **p < 0.01. i, j H3K18la levels in normal and tumor tissues. i H3K18la levels were visualized by immunofluorescence staining. Scale bar: left panel, 100 μm; right panel, 20 μm. j. Statistical results of H3K18la levels in normal and tumor tissues. Unpaired t test, **p < 0.01. k Kaplan-Meier curves of time for recurrence showing the difference between ocular melanoma patients with low and high H3K18la levels. “High H3K18la” was defined as relative lactylation level of the samples (determined by luminance intensity) higher than 0.53, while those lower than 0.53 as “low H3K18la”. n = 56, log-rank test, p < 0.05. l H3K18la levels in patients at AJCC stages T1 to T4. There were 3 ocular melanoma samples in T1 stage, 35 in T2 stage, 41 in T3 stage, and 3 in T4 stage. m Lactyation and H3K18la levels were detected in normal and tumor tissues by Western blot

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