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Table 1 Number of unique insertion sites (IS) identified via PCIP-seq

From: PCIP-seq: simultaneous sequencing of integrated viral genomes and their insertion sites with long reads

Sample name Virus Host PVL Template μg Raw reads Chimeric reads (%) Pure host / pure viral reads Insertion sites Largest clone (%)
ATL2 HTLV-1 HSA nd 4 81,219 68.21 0.0037 / 31.8 160 49.5
ATL100 HTLV-1 HSA 106 4 4838 64.14 9.16 / 26.7 13 89.624
233 BLV OAR 78.3 7 524,698 53.4 0.04 / 46.53 5311 5.22
221 (022016) BLV OAR 63 4 180,276 67.14 3.59 / 29.27 8023 0.625
221 (032014) BLV OAR 16 4 32,266 68.69 0.11 / 31.20 5374 0.279
220 BLV OAR 3.8 2 44,876 67.38 0 / 32.62 1352 3.55
1439 BLV BosT 45 3 181,055 70.52 0.19 / 29.29 5773 1.17
560 BLV BosT 0.644 1 6802 69.83 1.12 / 29.06 172 4.59
1053 BLV BosT 23.5 6 367,454 72.13 0.04 / 27.83 17,903 0.353
HIV_U1 HIV-1 HSA 200 2 94,086 54.66 2.75 / 42.59 728 47.2
Jurkat U1–0.1 HIV-1 HSA 0.2 5 252,913 43.33 0.04 / 56.62 4 71.7
Jurkat U1–0.01 HIV-1 HSA 0.02 5 234,421 43.33 0.04 / 56.52 2 90.2
Jurkat neg HIV-1 HSA 0 5 12,137 0 100 / 0 0 0
02006 HIV-1 HSA 0.46 12 240,641 51.63 1.10 / 47.27 158 7.82
06042 HIV-1 HSA 0.56 8 226,685 21.18 0.41 / 78.41 73 4.77
HPV18_PX HPV18 HAS nd 4 180,550 21.36 0.29 / 78.35 55 nd
HPV18_PY HPV18 HAS nd 4 82,807 0.09 0.05 / 99.86 19 nd
  1. Chimeric reads reads containing host and viral DNA, cover the integration site, Pure host/pure viral reads reads containing either host or viral DNA, do not include the integration site, Largest clone % insertion site with highest number of reads in that sample, PVL proviral load. (Percentage cells carrying a single copy of integrated provirus or number proviral copies per 100 cells)