Fig. 2

Base editing outcome and efficiency mapping for sgRNAs using genome-integrated target site library assay. a Schematic of the outcome and efficiency mapping from edited reads in integrated target sites. For coding regions, the outcome for an sgRNA was determined as the amino acid change with the highest fraction of reads. For noncoding regions, outcome was defined as the single base change of variants with the highest fraction of reads. b Editing frequencies at the integrated target sites in cells edited with AncBE4max or ABEmax, respectively. The orange line represents the median of the fraction of edited reads for all sgRNAs in the library. c, d Fraction of sgRNAs associated with different number of outcomes before and after merging outcomes at amino acid level after editing with AncBE4max (c) or ABEmax (d). e Distribution of the major outcome fraction after editing with AncBE4max and ABEmax. f, g Correlations of editing efficiencies at integrated target sites measured from the genome-integrated target site library assay and those at endogenous sites determined from amplicon sequencing for cells edited with AncBE4max (f) or ABEmax (g). Pearson’s correlation coefficients are shown