Fig. 6

On-target sequence fidelity at the Tspan2 CArG box. Percent editing across HDR (a) and PE2 (b) founder mice. CRISPResso sequence output for individual founders from sgRNA (c) and pegRNA (d) study. Protospacer (blue line) and PAM (red box) are indicated as are numbers indicating frequency of correct edits. Black boxes in panel c indicate deletions. Please note, a 150 bp deletion could not be aligned in CRISPResso for HDR founders 7, 10, and 33, but is included in the quantitative data of panel a. PE2 founders 32 and 38 represent littermate controls that did not exhibit on-target editing