Fig. 6

SPLiT-seq single-cell transcriptomic analysis of ACME-dissociated cells, overview, and metrics. a Experimental workflow. We used ACME-dissociated and FACS-sorted cells from the planarian species S. mediterranea and D. japonica, after two freezing steps. For SPLiT-seq, combinatorial barcoding consisted of 4 rounds of barcoding with 48 × 96 × 96 × 3 barcodes. cDNA molecules coming from each cell are uniquely labeled by one of the 1,327,104 possible barcode combinations. b Violin plots showing the distribution of UMI counts and genes detected per cell. c Saturation plots for UMIs per cell (left) and genes per cell (right) at given fractions of the complete sequencing depth, for the 19,741 and 14,086 single-cell transcriptomes sequenced above the threshold for S. mediterranea and D. japonica, respectively. d Scatter plot of S. mediterranea (red) vs D. japonica (blue) UMI counts per cell. Collisions are shown in gray