Fig. 5
From: ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics
Droplet-based single-cell transcriptomic analysis of ACME-dissociated cells from the cnidarian Nematostella vectensis. a Experimental workflow. We used ACME-dissociated and FACS-sorted G1 cells from the cnidarian species N. vectensis, after one freezing step. For droplet-based single-cell transcriptomic analysis, we used the 10X Genomics Chromium platform. b UMAP visualization of 3899 N. vectensis cells, colored by cluster identity and annotated on the basis of marker genes. c Violin plots showing the distribution of UMI counts and genes detected per cell. d Comparison of cell proportions for N. vectensis with a previous cell type atlas (Sebé-Pedrós et al.). Cell clusters are grouped by cell type