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Fig. 1 | Genome Biology

Fig. 1

From: ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

Fig. 1

ACME cell dissociation and fixation. a Whole dissociation process for the planarian Schmidtea mediterranea. From left to right: live worms used as input in water, ACME dissociation reaction after 10–60 min, cell suspension after final pipetting, pellet after first centrifugation, and pellet after second centrifugation in PBS 1% BSA. be Flow cytometry profiles of S. mediterranea ACME-dissociated cells stained with DRAQ5 (nucleus) and Concanavalin-A (cytoplasm): ungated (b), after gating singlets by FSC (c), and DRAQ5 (d). Area vs height, and resulting clean G1 (red) and G2 (cyan) populations. Singlets are selected based on their well-correlated area vs height signal, while aggregates display high area vs height ratios. f Relative proportion of singlets in a typical S. mediterranea ACME cell dissociation, corresponding to 22.8% of the total events, and histogram of their DNA content (linear scale), showing the relative proportions of G1 and G2 cells. g Bright field (BF) and confocal fluorescence (Fluo) microscopy images of S. mediterranea ACME-dissociated cells stained with Concanavalin-A and DRAQ5, showing single cells, aggregates, and debris (top) and details of different cell types with well-preserved morphology (bottom). Scale bars are 50 μm (top) and 5 μm (bottom)

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