TY - JOUR AU - García-Castro, Helena AU - Kenny, Nathan J. AU - Iglesias, Marta AU - Álvarez-Campos, Patricia AU - Mason, Vincent AU - Elek, Anamaria AU - Schönauer, Anna AU - Sleight, Victoria A. AU - Neiro, Jakke AU - Aboobaker, Aziz AU - Permanyer, Jon AU - Irimia, Manuel AU - Sebé-Pedrós, Arnau AU - Solana, Jordi PY - 2021 DA - 2021/04/08 TI - ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics JO - Genome Biology SP - 89 VL - 22 IS - 1 AB - Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and are sortable and permeable. As a proof of principle, we provide single-cell transcriptomic data of different species, using both droplet-based and combinatorial barcoding single-cell methods. ACME uses affordable reagents, can be done in most laboratories and even in the field, and thus will accelerate our knowledge of cell types across the tree of life. SN - 1474-760X UR - https://doi.org/10.1186/s13059-021-02302-5 DO - 10.1186/s13059-021-02302-5 ID - García-Castro2021 ER -