Addressing uncertainty in genome-scale metabolic model reconstruction and analysis

Table 5 Summary of approaches that address sources of uncertainty in flux simulation. Highlighted in bold are key approaches related to probabilistic or ensemble-based methods

Approach	Sources of uncertainty	References
Alternative objective functions	Undefined cellular objective	[130,131,132, 134, 135]
Suboptimal solutions	Undefined cellular objective	[136,137,138]
Characterization of optimal solutions	Degenerate otimal solutions	[141,142,143,144,145]
Characterization of steady-state solution space	Degenerate solution space	[146,147,148,149]
Random sampling	Degenerate solution space	[151,152,153,154,155,156,157,158,159,160]
Random sampling with probabilistic biases	Degenerate solution space	[161, 162, 165]
Added constraints	Degenerate solution space	[55, 168,169,170,171,172,173,174, 177,178,179, 182,183,184,185]
Relaxed steady-state assumption	Steady-state assumption	[188, 189]

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