Fig. 2

Benchmark of MinLibCas9 library. a Standardised area under the receiver operating characteristic curve for 245 cell lines at 20% FDR for the essential genes calculated using the minimal and original full library. b Average Precision (AP) scores to classify significant gene dependencies identified at 1% FDR in Project Score library using gene fold-changes from MinLibCas9. Recall-Precision curves for all cell lines are represented in the inset and cell lines with the lowest and highest AP score are highlighted. c, CRISPR-Cas9 screen performed on HT-29 cancer cells using MinLibCas9 library. d Correlation between gene-level fold-changes obtained with the two libraries. e Recall of essential genes across all replicates for HT-29 performed with MinLibCas9 and Project Score libraries