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Fig. 1 | Genome Biology

Fig. 1

From: Minimal genome-wide human CRISPR-Cas9 library

Fig. 1

Genome-wide human CRISPR-Cas9 sgRNA libraries. a Number of sgRNAs in each CRISPR-Cas9 library since the first reported genome-wide screens, excluding Wang et al. [3] which targets 7114 genes. b Area under the recall curve of sgRNAs targeting known essential (n = 1469) and non-essential (n = 3251) genes, and non-targeting guides (n = 997). Recall curves were calculated for each replicate of Project Score [13] (n = 663) and represented by the cumulative distribution of each sgRNA group across all sgRNAs sorted by ascending fold-changes. Box-and-whisker plots show 1.5× interquartile ranges and 5–95th percentiles, centres indicate medians. c Fold-change distribution, based on Project Score data-set of the different sgRNAs groups. Diagram depicting how the KS scores vary across the CRISPR-screens fold-change range. KS scores are calculated by testing if the distribution of each sgRNA across cell lines is drawn from that of the non-targeting sgRNAs using a two-sided Kolmogorov-Smirnov distribution

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