Fig. 5

CIT regulates the cytoskeletal and microtubule network in GC cell lines. a Phosphoproteins detected in YCC3 cells following CRISPRi-mediated suppression of CIT expression (results are shown as an average of two CIT sgRNAs in duplicate). b Validation of NDRG1 phosphorylation regulated by CIT in YCC3 cells. Seven DPI with CIT sgRNAs WB analysis was used to quantify the protein levels of CIT, NDRG1 and phspho-NDRG1 (Thr346). c Compound classes that score as associated with the CIT expression signature using the L1000 platform [29]. d Individual compounds within drug classes that score as associated with CIT signature. e CIT-dependent or CIT-independent cell lines were treated with PKC activators, and proliferation was assessed after 7 days using a crystal violet staining assay. f Images of crystal violet staining following treatment with PKC activators