Fig. 2

Characteristics of Cas13d-mediated circRNA knockdown. a Optimized Cas13d system targeting 8 circRNAs, each with 2 gRNAs. qRT-PCR for circular and linear transcripts after knockdown of circRNAs in Huh7 cells. b Top, schematic drawing of circEGFP-targeting guide RNA sequence and spacer position-matched shRNA. Bottom, relative circEGFP knockdown by individual position-matched gRNA and shRNA. NT, non-targeting. c Volcano plots of differential transcript levels between circEGFP-targeting and non-targeting shRNAs (left) or circEGFP-targeting CasRx and non-targeting guide (right) as determined by RNA sequencing. d Summary of significant off-target transcript perturbations by matched Cas13d gRNA and shRNA. The data shown are from one of two biological replicates with similar results, and error bars indicating the mean ± s.d. of three technical replicates