Fig. 2

Characteristics of Iso-seq transcripts. a Examples of alternative splicing (CD44) and alternative promoter (ERBB2) events identified in the Iso-seq data. Exon orders are labeled at the bottom, and overlapping exons are indicated by alphabets. For ERBB2, only overlapping exons are labeled. Novel exons (relative to Gencode annotation) were indicated in black. Protein domain annotation for selected isoforms was shown in the lower panel. b Characteristics of novel (NIC and NNC) and known (FSM) transcripts. NIC and NNC have more exons and longer isoforms and CDS. Novel transcripts can generate novel TSS and TTS. c Novel isoforms are more likely to be target of NMD, expressed at lower level and contain more MHC binding sites than known isoforms