Fig. 2

Whole genome resistance profiling identifies known and novel targets/mechanisms of action. a The top hit genes (FDR < 0.1) identified from each drug screening using MAGeCK. A subset of genes previously linked to modulate drug sensitivity (red text). b A CRISPR-Cas9 knockout screen identified PPP6C required for docetaxel cytotoxicity. CRISPR-Cas9-targeted cells decreased their sensitivity to docetaxel in HAP1 and HeLa cells. c NAA60 is involved in methotrexate sensitivity. CRISPR-Cas9-targeted cells decreased their sensitivity to methotrexate in HAP1 and HeLa cells. d A putative small-molecule transporter (SLC43A2) for oxaliplatin. CRISPR-Cas9-targeted cells decreased their sensitivity to oxaliplatin in HAP1 and HeLa cells