Fig. 2

Inflammatory response mRNAs are selectively translated in G0 leukemic cells, where canonical translation is inhibited. a Repression of canonical translation. b Polysome profiles of S+, SS, and AraCS are shown. Polysome-associated mRNAs were isolated and analyzed by microarray. Graph of polysome to monosome (P/M) ratios in S+, SS, and AraCS. c, d Western analysis of translation initiation regulators: c eIF2α, phospho-eIF2α, and its regulators phospho-PERK and phospho-PKR, and d of translation regulator, eIF4EBP (4EBP) at Thr37/40 and Ser65 phosphorylation sites and total levels with quantification below. e Number of differentially expressed genes. f Venn diagram of transcriptionally and translationally upregulated genes in G0 cells induced by AraC and SS, compared to S+ cells, is shown on the left. Heatmap of gene expression changes at the transcriptome, translatome, and ribosome occupancy (RO) levels is shown on the right. See also Additional file 2: Table S1 for the 490 translationally upregulated genes and their RO changes. g Gene ontology (GO) analyses of differentially expressed genes shown in Fig. 2e. Statistical significance of enriched GO categories is shown as a heatmap. h Expression of signature genes of G0 leukemic cells in published transcriptomes of in vivo resistant leukemic and G0 models. i Translatome analysis of G0 cells from five different cell types. Heatmap of normalized enrichment score (NES) is shown. *p ≤ 0.05. Data are represented as average ± SEM. See also Additional file 1: Figures S1, S2 and Additional file 2: Table S1