Skip to main content
Fig. 1 | Genome Biology

Fig. 1

From: Opportunities and challenges in long-read sequencing data analysis

Fig. 1

Overview of long-read analysis tools and pipelines. a Release of tools identified from various sources and milestones of long-read sequencing. b Functional categories. c Typical long-read analysis pipelines for SMRT and nanopore data. Six main stages are identified through the presented workflow (i.e. basecalling, quality control, read error correction, assembly/alignment, assembly refinement, and downstream analyses). The green-coloured boxes represent processes common to both short-read and long-read analyses. The orange-coloured boxes represent the processes unique to long-read analyses. Unfilled boxes represent optional steps. Commonly used tools for each step in long-read analysis are within brackets. Italics signify tools developed by either PacBio or ONT companies, and non-italics signify tools developed by external parties. Arrows represent the direction of the workflow

Back to article page