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Fig. 5 | Genome Biology

Fig. 5

From: Genome-wide MNase hypersensitivity assay unveils distinct classes of open chromatin associated with H3K27me3 and DNA methylation in Arabidopsis thaliana

Fig. 5

Functional validation of sMHSs using an agrobacterium-mediated transient assay. a The relative potential of 21 sMHSs in transcriptional regulation. N. benthamiana leaves were transiently transformed using Agrobacteria containing constructs of sMHS-mini35S::LUC. sMHS1-sMHS10 represent the 10 sMHSs associated with H3K27me3. sMHS11-sMHS21 represent the 11 sMHSs not associated with H3K27me3. L3 and C4 were validated as strong enhancers in leave, and N1 has no function to drive gene expression in a previous study [28]. y-axis represents the fold enrichment of luciferase signals of each construct compared to a construct containing only the mini35S promoter. b A representative N. benthamiana leaf infiltrated with different constructs. Color scale represents the luminescent signal intensity measured by cps (counts per second)

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Genome Biology

ISSN: 1474-760X

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