Fig. 3

DNase I and Tn5 sensitivity and nucleosome occupancy associated with MHSs. a DNase I and Tn5 sensitivity at cMHSs and sMHSs. All cMHSs and sMHSs were aligned at the midpoints and the average DNase I sensitivity at each base pair spanning ± 1 kb from the midpoint was plotted. Blue and red line represent DNase I sensitivity of cMHSs and sMHSs, respectively. Green and orange lines represent Tn5 sensitivity at cMHSs and sMHSs, respectively. b Heatmaps of nucleosome occupancy spanning ± 1 kb from the midpoints of cMHSs and sMHSs, respectively. Nucleosome occupancy was calculated by deepTools. Each line represents nucleosome occupancy of one cMHS or sMHS. cMHSs and sMHSs were sorted by length in the plot. c Distribution of H3K27me3 and H3K27ac at MHSs. Genomic regions ± 1 kb from the midpoints of MHSs were divided into 10-bp bins and average number of H3K27me3 and H3K27ac reads in each bin were calculated