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Fig. 1 | Genome Biology

Fig. 1

From: Genome-wide MNase hypersensitivity assay unveils distinct classes of open chromatin associated with H3K27me3 and DNA methylation in Arabidopsis thaliana

Fig. 1

Identification of open chromatin based on MH-seq. a A schematic diagram of MH-seq. Chromatin is lightly digested by MNase. MNase cuttings at linkers generate large fragments (> 150 bp) which are protected by nucleosomes. MNase cutting at open chromatin regions generates small fragments (< 100 bp) which are associated with regulatory proteins. The small DNA fragments are collected for library construction and sequencing. b A representative open chromatin region on chromosome 5 based on MH-seq. Profiles from ATAC-seq and DNase-seq data (single-cut and double-cut) are included for comparison. Open chromatin regions are marked by black rectangle under profile of each technique. Gene models are shown at the bottom

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