Fig. 4

Schematic representation of genomic regulatory features associated with differentially spliced AS events. For exons (a), introns (b), alternative donor (c), and acceptor (d) sites belonging to any of the three AS core sets, arrows summarize which features show statistically significant differences with respect to the Genome set of events and the direction of these differences (higher or lower). “X” indicates no statistically significant difference. Features include length of the alternatively spliced sequence together with its downstream and upstream exons/introns, GC content, maximum entropy (MaxEnt) score for 5′ and 3′ splice sites (ss strength), and distance between the branch point and the 3′ splice site (BP-AG distance). Differences are indicated separately for events for which the inclusion of the alternative sequence is down- (red) and up- (blue) regulated in the core sets. The genome set contains events fulfilling the read coverage criteria used for AS analyses. Genomic features were extracted using Matt [49]. ss, splice site. e Scatterplot of ΔPSI values for IR and ES events belonging to the abiotic stress AS core set whose inclusion is affected (|ΔPSI| >15) by the studied splicing factors. ΔPSI between abiotic and control conditions corresponds to the average ΔPSI of experiments in which the event was found to change significantly. p values correspond to Binomial tests for quadrants I and III vs. the total