Fig. 6
From: Disome-seq reveals widespread ribosome collisions that promote cotranslational protein folding
The cryo-EM structure of disomes. a A composite map of the cryo-EM structure of disomes. The disomes were collected without cycloheximide in the lysis buffer. Both the leading and the trailing ribosomes are in the rotated state harboring hybrid A/P (blue) and P/E tRNAs (green). Small subunits are labeled by orange and yellow, and large subunits are labeled by cyan and gray. A model of mRNA is highlighted in red. b The top views on disomes (top) and di-ribosomes (middle). The structure of di-ribosomes was from Ikeuchi et al. [9]. An overlay is shown (bottom) by docking di-ribosomes to disomes, aligned according to the trailing ribosomes; the conformational difference in the leading ribosomes is highlighted by the arrow. c–e The zoomed-in detail of the contact interface between the 40S subunits of the leading and the trailing ribosomes, in particular, the 40S head-to-head contact site of disomes (d) and that of di-ribosomes (e). The blue star indicates a strong interaction between the two ribosomes in di-ribosomes, and the red crosses indicate the missing interactions in disomes