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Table 6 HG002 assembly statistics for the Flye and HiCanu assemblies as well as the Ash1 reference assembly. Misassemblies are filtered to exclude errors in known SVs and segmental duplication sites as well as centromeric, telomeric, and gap regions. All metrics are computed by QUAST except k-mer completeness and quality value which are computed by Merqury. Best results are highlighted

From: Ratatosk: hybrid error correction of long reads enables accurate variant calling and assembly

 

ONT Flye

ONT+Ratatosk Flye

PacBio HiFi HiCanu

Ash1

Reference coverage (%)

94.90

95.85

96.71

98.50

k-mer completeness (%)

95.79

97.27

97.45

97.67

Quality value

33.97

47.90

55.17

41.341

N50 (Mbp)

37.98

45.05

44.67

34.30

NA50 (Mbp)

20.83

25.46

19.78

16.48

# contigs

972

430

422

2,412

# misassemblies

68

75

84

188

# mismatches / 100 kbp

121.71

112.66

178.36

161.09

# indels / 100 kbp

109.67

26.66

26.84

27.00