Fig. 5

Reproductive 21PHAS precursors are widely cleaved by miR2118 on membrane-bound polysomes in maize and rice. a, c Abundance of different miR2118 members in input (Total), TP, and MBP samples from maize immature tassels (a) and rice immature panicles (c). b, d Number (left panels) and overlap of 21PHAS precursors cleaved by miR2118 (right panels) in Total, TP, and MBP samples from maize immature tassels (b) and rice immature panicles (d). Cleavage sites with category = 0, which means that the reads with the maximum count (> 1) are mapped to only one indicated position on the transcript, and P value ≤ 0.05 in at least two biological repeats were filtered as miR2118 target sites. e, f Abundance of reproductive 21-nt phasiRNAs in Total, TP, and MBP samples from maize immature tassels (e) and rice immature panicles (f). Abundance of miR2118 and reproductive 21-nt phasiRNAs (a, c, e, and f), and number of target 21PHAS precursors (left panels of b and d) are displayed as mean ± standard deviation (SD) of three biological repeats. “RPMR” is short for “reads per million rRNA fragments” (a, c, e, and f). Comparisons between MBP and TP (a and c), and between Total, TP, and MBP (left panels of b and d, e and f) were performed by two-tailed unpaired t-test. P values are displayed to the right of the corresponding MBP miR2118s (a and c) or above the corresponding comparisons (left panels of b and d, e and f)