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Fig. 2 | Genome Biology

Fig. 2

From: Widespread occurrence of microRNA-mediated target cleavage on membrane-bound polysomes

Fig. 2

Retrotransposons and DNA transposons contribute differentially to polysome association of TE-derived siRNAs in maize. a Identification of 22-nt TE-siRNAs that were differentially accumulated between TP and input (Total) (left panel), and between MBP and TP (right panel) in maize immature tassels. b Identification of 24-nt TE-siRNAs that were differentially accumulated between TP and Total (left panel), and between MBP and TP (right panel) in maize immature tassels. TE-siRNA abundance is displayed as the mean of three biological repeats. “RPMR” is short for “reads per million rRNA fragments”. c Overlap of TE loci that generated differentially accumulated siRNAs in the various comparisons in a and b. d Contributions of retrotransposons and DNA transposons to the differentially accumulated 22-nt and 24-nt TE-siRNAs in maize immature tassels. “Genome-wide” denotes the contributions of the transposon types to total TE-siRNAs. LINE: long interspersed nuclear element; LTR: long terminal repeat; SINE: short interspersed nuclear element; TIR: terminal inverted repeat. The cutoff parameters for differentially accumulated TE-siRNAs are fold change ≥ 2 and P value ≤ 0.05. “Group I,” “Group II,” “Group III,” and “Group IV” represent “TE loci with 22-nt or 24-nt siRNAs that were polysome-depleted,” “TE loci producing 22-nt or 24-nt siRNAs that were polysome-associated,” “TE loci with 22-nt or 24-nt siRNAs that were likely on free polysomes (FPs),” and “TE loci with 22-nt or 24-nt siRNAs enriched on MBPs,” respectively

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