Fig. 4
Quantitative profiling of m6A in stem-differentiating xylem of Populus trichocarpa. a Flowchart of DRS library construction and sequencing. b The distribution of m6A sites across the transcripts. c Comparison of m6A sites from two independent biological repeats. d Box plot shows DRS reads coverage of overlapped m6A sites and repeat2-specific m6A sites. e All the RRACH motif methylation supported by Nanopore DRS reads. f The m6A ratio for all the modified genes and wood formation-related genes. g The correlation between m6A ratio and gene expression. h Venn diagram shows the overlap between m6A sites predicted with Nanom6A and Epinano. i The distribution of m6A site for overlapped sites, Nanom6A-specific sites, and EpiNano-specific sites. j The m6A ratio for overlapped sites and Nanom6A-specific sites. k The modification sites of ARK2 from DRS, EpiNano, MINES, and MeRIP-Seq. Blue and red colors in MeRIP-Seq represented Input and IP library, respectively