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Fig. 2 | Genome Biology

Fig. 2

From: Quantitative profiling of N6-methyladenosine at single-base resolution in stem-differentiating xylem of Populus trichocarpa using Nanopore direct RNA sequencing

Fig. 2

Validation of Nanom6A using known m6A sites in mammal. a Strategies to identify m6A site from ACTB site (1216) using ionic current signals in two DRS reads from wild-type and shMETTL3, respectively. b Bar plot shows the predicted m6A ratio from wild-type and shMETTL3, respectively. c Venn diagram of the number of predicted m6A sites from wild-type and shMETTL3, respectively. d Bar plot displays the consistency between known m6A sites based on the SCARLET method and Nanom6A prediction. The x-axis represents the modified sites in TUG1. The y-axis shows the number of modified reads. e Three bar plots show consistency between known modified sites in ACTB and Nanom6A prediction, enrichment of MeRIP-Seq IP over input reads, and Nanom6A-modified ratio, respectively. f Bar plots demonstrate consistency between known modified sites in BSG and Nanom6A prediction, enrichment of MeRIP-Seq IP over input reads, and Nanom6A-modified ratio, respectively

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