Skip to main content

Table 2 Peptide sequences tested using ELISpot assay

From: Aberrant splicing isoforms detected by full-length transcriptome sequencing as transcripts of potential neoantigens in non-small cell lung cancer

Peptide sequence gene NetMHC score Isoform-detected samples Isoform/frameshift GENCODE isoform type
TYMAGSEAW NDST1 0.0327 Case 6 Frameshift Frameshift Frameshift
VWPTAWASL NDST1 0.1467 Case 6 Frameshift Frameshift Frameshift
RYCRGVMLL ARL8B 0.2162 Case 4 Frameshift Frameshift Frameshift
SFTDISIYL SENP2 0.3557 Case 5 Frameshift Frameshift Frameshift
KFSPEPSQF CEACAM6 0.0565 Case 4 Isoform Novel Alternative last exon
SFPLVFLFF CEACAM6 0.0857 Case 4 Isoform Novel Alternative last exon
NYFNLGMVV ERO1A 0.4236 Case 2 Isoform Novel Alternative last exon
TYTTIKINF HOOK2 0.0185 ABC-1, H1437, H1648, H1650, H2126, H322, II-18, PC-3, PC-9, RERF-LC-Ad1, RERF-LC-Ad2, RERF-LC-KJ, RERF-LC-OK, Case 6, Case 7, VMRC-LCD Isoform ENST00000589134 Alternative last exon
AWPKHLDLM MCEE 0.3848 ABC-1, H2126, H322, II-18, PC-3, RERF-LC-KJ, Case 1, Case 7 Isoform Novel Alternative 5′ splice site
VYFTSDFKV PKM 0.2639 ABC-1, H1299, H1437, H1648, H2126, H2347, H322, II-18, RERF-LC-Ad1, RERF-LC-Ad2, Case 4, VMRC-LCD Isoform Novel Alternative last exon
SFLGFSILL PKM 0.3814 ABC-1, H1299, H1437, H1648, H2126, H2347, H322, II-18, RERF-LC-Ad1, RERF-LC-Ad2, Case 4, VMRC-LCD Isoform Novel Alternative last exon
QYSLATAFL SCGB3A2 0.2403 Case 6 Isoform Novel Unannotated exon
SYFETIAAL SELENBP1 0.0848 Case1, Case 4, Case 6 Isoform ENST00000493560 Intron retention
RFQPHGDGW TMC4 0.2835 H322, RERF-LC-Ad2, Case 1 Isoform Novel Alternative last exon
TWLTSGPHL TMEM45A 0.2974 Case4, Case7 Isoform Novel Combination (unannotated exons)
FLLPAPFPF TUFM 0.3991 A427, ABC-1, H1299, RERF-LC-Ad1, RERF-LC-Ad2, Case 1, VMRC-LCD Isoform Novel Intron retention
KYEEVAQLY UQCRB 0.447 H1819, Case 2, Case 3 Isoform ENST00000519322 Alternative last exon