Fig. 3

The presence of eArray expands the compacting ability of SGC. a The design and content of eArray. The 10 essential genes assembled in eArray distribute across 7 loci (a–g) in the first 140 kbp of synXIIL. Locus d is shown in detail with sequences that were copied from synXIIL to eArray. Other loci are defined similarly, except for the left end of locus b, in which only 110 bp are used to avoid the inclusion of complete YLL037W open reading frame. Homologous sequences (shown in blue) were added to these 7 loci by PCR. PCR products and a linearized vector were co-transformed into yeast to assemble eArray. The genes encoded and other functional elements in the eArray are shown at the bottom. The centromere-containing sequences are shown in black. b Plating results of SGC in strains with or without eArray. The URA3 reporter was integrated between the essential gene GPI13 and nonessential gene YLL032C in LU-20 in these two strains before SGC. Equal amount of cells were plated after SGC. c The LU profiles of selected 5-FOA resistant strains after SGC. ZLY307 with the URA3 reporter in LU-24 and eArray was SCRaMbLEd and plated onto 5-FOA plates. This figure uses the same design strategy as Fig. 2a. d A box plot showing the distribution of number of LUs deleted in each strain in the group with eArray (+) in b and the group without eArray (−) in Fig. 2e. e A box plot showing the distribution of lengths of deleted sequences in each strain in the group with eArray (+) in b and the group without eArray (−) in Fig. 2e. f A box plot showing the distribution of numbers of genes deleted in each strain in the group with eArray (+) in b and the group without eArray (−) in Fig. 2e