Fig. 2

Compacting the left arm of synthetic chromosome XII (synXIIL) by SGC method. a The LU profiles of selected 5-FOA resistant strains after SCRaMbLEing the synXIIL strain with URA3 reporter in LU-8 (ZLY292). Nonessential LUs are shown in gray, essential-gene-containing LUs are shown in red, and centromere-containing LU are shown in brown. Deleted LUs are shown in white. The position of URA3 integration is marked by a hollow triangle. b The numbers of LUs deleted in strains in a. The average number of deleted LUs is also shown. c The lengths of deleted sequences in strains in a. The average length of deleted sequences is also shown. d The numbers of deleted genes in strains in a. The average number of deleted genes is also shown. e–h The LU profiles of selected 5-FOA strains after SCRaMbLEing the synXIIL strain with the URA3 reporter in LU-24 (ZLY293). The URA3 reporter was integrated at indicated sites by transforming the last chunks of megachunk A (for LU-8) or megachunk C (for LU-24), the fragments we used in our previous publication [23]. The integration of the last chunk of megachunk C introduces two additional loxPsym sites into LU-24 and divides LU-24 into three small LUs, 24-a, 24-b, and 24-c, which are indicated by the black line