Fig. 1
From: SpRY greatly expands the genome editing scope in rice with highly flexible PAM recognition
Analysis of SpG nuclease activity on different NGN PAMs in transgenic rice. a The gene construct of SpG nuclease used for genome editing in transgenic rice. Ubi-P, maize ubiquitin 1 promoter; NLS, nuclear localization signal. b Summary of the genotyping results on T0 transgenic rice lines generated with SpG and SpCas9-NG nuclease. c Comparison of the mutation frequency by SpG and SpCas9-NG nucleases toward 4 NGN PAMs in T0 transgenic rice lines