Fig. 1

Variations of promoter H3K27ac levels among MYCN-amplified NBL samples. a–c Promoters were classified as active or inactive based on H3K27ac levels in individual NBL tumors: a NBL cell line samples, b NBL O-PDX samples, and c NBL cell line and O-PDX samples. Each promoter region plotted spans TSS ± 5000 bp binned by non-overlapping 250-bp windows; the color bar represents the scaled windowed H3K27ac enrichment, from 0 (lowest) to 3 (highest). Horizontal dotted lines delineate shared promoters (active in all samples) and sample-specific promoters (promoter activity in at least one sample was different from the remaining samples). Promoters were sorted by average descending H3K27ac enrichment across all samples within each group. d, e Venn diagram indicating the number of shared and sample-specific H3K27ac promoter activities between NBL cell line samples (d) and O-PDX samples (e). f The promoter activity variation is highlighted by the proportion of sample-specific active promoters among all H3K27ac active promoters, as compared within cell line or O-PDX NBL samples, and between cell line and O-PDX NBL samples